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Image Search Results
Journal: Frontiers in Oncology
Article Title: Molecular features and predictive models identify the most lethal subtype and a therapeutic target for osteosarcoma
doi: 10.3389/fonc.2023.1111570
Figure Lengend Snippet: SQLE knockdown produced significant inhibition on cancer-related phenotypes of U2OS cells in vitro . (A) Quantitative PCR (Q−PCR) with mRNA expression confirmed the knockdown of SQLE after 24h siRNA transfection. (B) Western blot analyses with protein expression confirmed the knockdown of SQLE after 48h siRNA transfection. The β-actin was treated as the loading control. (C) Cell proliferation was suppressed by SQLE knockdown. (D) Cell colony formation ability was reduced by SQLE knockdown. (E) Cell migration was inhibited by SQLE knockdown. The scale bar represented 100 μm. (F) and (G) indicated the quantification of positive signals from (D, E) , respectively. (H) The knockdown of SQLE significantly reduced the intracellular cholesterol. Error bars represented ± SD of three biological replicates. **P < 0.01, ***P < 0.001 by Student t-test.
Article Snippet: The
Techniques: Produced, Inhibition, In Vitro, Real-time Polymerase Chain Reaction, Expressing, Transfection, Western Blot, Migration
Journal: Frontiers in Oncology
Article Title: Molecular features and predictive models identify the most lethal subtype and a therapeutic target for osteosarcoma
doi: 10.3389/fonc.2023.1111570
Figure Lengend Snippet: Terbinafine treatment impaired the growth and migration of U2OS cells in vitro . (A) Different gradient concentrations of terbinafine were added to the U2OS cells. As the dosage of terbinafine increased, the viability of the cells decreased. (B) The U2OS cells were respectively treated with 25 μM and 50 μM terbinafine. Cell colony formation ability was found reduced with the increasing dosage of terbinafine. (C) The number of migrating cells decreased obviously with the increase in drug concentration. Scale bars, 100 μm. (D, E) indicated the quantification of positive signals from (B, C) , respectively. (F) The terbinafine treatment significantly reduced the intracellular cholesterol. Error bars represent mean ± SD; statistical analysis was performed using the Student t-test. **P < 0.01, ***P < 0.001.
Article Snippet: The
Techniques: Migration, In Vitro, Concentration Assay
Journal: PLoS ONE
Article Title: Genomic Instability of Osteosarcoma Cell Lines in Culture: Impact on the Prediction of Metastasis Relevant Genes
doi: 10.1371/journal.pone.0125611
Figure Lengend Snippet: Number of differentially regulated genes enriched in KEGG pathways after serial passaging of indicated cell lines.
Article Snippet: Human SAOS (HTB-85), HOS (CRL-1543) and
Techniques: Passaging
Journal: PLoS ONE
Article Title: Genomic Instability of Osteosarcoma Cell Lines in Culture: Impact on the Prediction of Metastasis Relevant Genes
doi: 10.1371/journal.pone.0125611
Figure Lengend Snippet: Number of differentially regulated genes enriched in KEGG pathways in high and low metastatic cell lines.
Article Snippet: Human SAOS (HTB-85), HOS (CRL-1543) and
Techniques:
Journal: PLoS ONE
Article Title: Genomic Instability of Osteosarcoma Cell Lines in Culture: Impact on the Prediction of Metastasis Relevant Genes
doi: 10.1371/journal.pone.0125611
Figure Lengend Snippet: Indicated genes are up-regulated (>2-fold; p<0.05) in at least three passage comparisons. Genes up-regulated in four passage comparisons are shown in bold. Genes marked in red are up-regulated in all three metastatic cell lines. Genes marked in purple, blue and green are shared by LM5 and LM8, LM5 and143B and LM8 and 143B, respectively.
Article Snippet: Human SAOS (HTB-85), HOS (CRL-1543) and
Techniques: